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Electrotransformation of Lactobacillus delbrueckii subsp. We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Using this procedure, we identified several plasmids able to replicate in L.
These vectors provide a good basis for developing molecular tools for L. Use of PCR-based methods for rapid differentiation of Lactobacillus delbrueckii subsp. We evaluated antioxidative effect of two antioxidative strains, isolated from the traditional fermented dairy products. Both intact cells and cell-free extract of Lactobacillus casei subsp. The cell-free extract showed excellent scavenging superoxide anion and hydroxyl radicals activity.
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The Cinac system and flow cytometry were relevant to distinguish different physiological states throughout growth. At the end of storage, Lactobacillus bulgaricus and L. Further genotypic analyses on representative strains from both taxa demonstrated that L.
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A comparison was made also between d0 and d10 in group B to quantify the efficacy of the 4 probiotics administered for 10 days. Interestingly, infection of enterocytes with B. The new policies did not immediately catch the attention of the populac To increase the productivity of lactic acid, a co-culture of lactobacilli was made by mixing 1: Multilocus sequence typing reveals a novel subspeciation of Lactobacillus delbrueckii.
The isolation and sequencing of pMC1 integration sites from these bacteria showed that in lactobacilli, pMC1 integrated into the conserved tRNA Ser gene.
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